Human 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1 (PLCB1) ELISA Kit

A sandwich ELISA from Assay Genie for the quantitative measurement of human 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1 (PLCB1) in biological samples. The assay is reactive with human samples and validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Reported performance includes a detection range of 0.312-20ng/ml and a sensitivity of 0.145ng/mL. Precision is 4.7% intra-assay CV and 8.1% inter-assay CV. The antibody pair is specific for natural and recombinant human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1. Recovery averages 91% (range 85-97) in serum and 93% (range 87-99) in plasma, with dilution linearity within 85-123% across serum, EDTA plasma and heparin plasma at 1:2 to 1:16 dilutions.

PLCB1 is a key signalling enzyme that mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Dysregulation of PLCB1 has been implicated in cancer, immune disorders and neurological conditions, and the target is listed under the cardiovascular research area. The associated UniProt accession is Q9NQ66. For research use only.