HSP27 (Phospho-Ser82)Colorimetric Cell-Based ELISA Kit

This cell-based ELISA kit from Assay Genie is designed for the quantitative measurement of phosphorylated HSP27 at Ser82 in cultured cells, using a convenient, lysate-free, high-throughput indirect ELISA format. The assay captures HSP27 (Phospho-Ser82) with phospho-specific primary antibodies; HRP-conjugated secondary antibodies then catalyze a colorimetric reaction upon substrate addition. The kit is reactive with Human, Mouse, and Rat samples, with a dynamic range of > 5000 cells and detection at 450 nm. It is intended for adherent cells and supports screening of the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on HSP27 phosphorylation.

Because the assay is qualitative in nature, two normalization strategies are provided: a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density so that results can be analysed by normalizing absorbance values to cell amounts. The phospho-specific design makes this kit relevant for studying the role of HSP27 phosphorylation in the cellular stress response and for research areas such as immunology, as well as broader investigation of HSP27 biology in contexts including cancer progression and neurodegenerative disorders.