HSL Colorimetric Cell-Based ELISA Kit

A cell-based immunoassay from Assay Genie measures hormone-sensitive lipase (HSL) levels in cell culture samples. The kit supports quantification of HSL across multiple cell types, with applications in research into lipid metabolism, energy homeostasis, and metabolic disorders including obesity, diabetes, and cardiovascular disease.

The lysate-free, high-throughput format detects HSL protein expression profiles in cultured cells, allowing measurement of relative HSL amounts and screening of treatments, inhibitors (including siRNA or chemicals), or activators. HSL is captured by HSL-specific primary antibodies; HRP-conjugated secondary antibodies then catalyse a colorimetric reaction upon substrate addition, read at 450 nm. The assay is suitable for adherent cells and is reactive with Human, Mouse, and Rat samples, with a dynamic range of > 5000 Cells. A monoclonal GAPDH antibody is included as an internal positive control, and Crystal Violet whole-cell staining determines cell density for normalisation. Storage is at 4°C for 6 Months.

HSL (gene symbol LIPE; UniProt Q05469) is a lipolytic enzyme of the 'GDXG' family that plays a rate-limiting role in triglyceride lipolysis, hydrolysing stored triglycerides to free fatty acids in adipose tissue and heart, and converting cholesteryl esters to free cholesterol in steroidogenic tissues. The Molecular Weight of the target is 84,128 Da, and the research area is Metabolism.