HSF1 (Phospho-Ser121)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the precise measurement of HSF1 phosphorylation at Ser121 in cell samples. HSF1 (Heat Shock Factor 1) is a key transcription factor involved in the cellular response to stress, and phosphorylation at Ser121 is critical for its activation and function. The kit enables researchers to detect and quantify phosphorylated HSF1 in cultured cells, and is suited to studying the mechanisms underlying stress response, cell survival, and disease progression, with applications in cancer biology, neurodegenerative disorders, and cellular stress pathways.

The assay uses an indirect ELISA format in which the target is captured by HSF1 (Phospho-Ser121)-specific primary antibodies, with HRP-conjugated secondary antibodies catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density. Detection is by colorimetric measurement at 450 nm, with a dynamic range of > 5000 cells. The kit is reactive with human and mouse samples and is categorised under the Epigenetics and Nuclear Signaling research area.