HRH1 Colorimetric Cell-Based ELISA Kit

Designed by Assay Genie for the quantitative detection of HRH1 (Histamine H1 Receptor) levels in cell lysates and tissue homogenates, this colorimetric cell-based ELISA kit offers high sensitivity and specificity for accurate and reproducible research results. HRH1 mediates the effects of histamine, a neurotransmitter involved in allergic reactions, inflammatory responses, and immune regulation, and its dysregulation has been implicated in allergies, asthma, and autoimmune diseases.

This lysate-free, high-throughput assay kit detects HRH1 protein expression profiles in cells via an indirect ELISA format. HRH1-specific primary antibodies capture the target while HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition. The kit is supplied in a microplate format with a dynamic range of > 5000 Cells, colorimetric detection at 450 nm, and storage at 4°C for 6 months. A monoclonal antibody specific for human GAPDH serves as an internal positive control, and a Crystal Violet whole-cell staining method is included to determine cell density for normalization. Reactivity is human.

Database identifiers listed with the product include Gene ID 3269 and UniProt ID P35367.