HOXD12 Transcription Factor Activity Assay

The HOXD12 Transcription Factor Activity Assay from Assay Genie is a non-radioactive DNA-binding ELISA that quantifies functional binding of the HOXD12 homeobox transcription factor to its consensus oligonucleotide sequence immobilized on a microplate. Nuclear extracts are added to wells pre-coated with a double-stranded HOXD12 response element; bound protein is then detected with a specific antibody and developed for colorimetric readout. The kit provides reagents for 96 Assays and is validated for Human, Mouse, and Rat nuclear and cell lysates prepared under standard extraction protocols. Researchers investigating limb morphogenesis, axial skeleton patterning, and HOXD-cluster gene regulation can apply this assay to compare transcription factor activity across developmental stages, tissue types, or experimental treatments. Store kit components refrigerated and protected from light until first use; intended for research use only.