HOXB2 Transcription Factor Activity Assay

HOXB2 (Homeobox B2) is a member of the HOX transcription factor family that plays key roles in vertebrate embryonic patterning, hindbrain segmentation, and hematopoietic differentiation. This Assay Genie DNA-Binding ELISA Kit quantifies active HOXB2 binding to its consensus DNA response element in nuclear and cell lysates, providing a functional readout of transcription factor activity rather than mere expression level.

The assay is validated for reactivity with human, mouse, and rat samples, supporting cross-species studies in developmental biology, stem cell research, and leukemia model systems. Each kit provides reagents for 96 reactions in a microplate format with a colorimetric readout, and components should be kept refrigerated to preserve antibody and oligonucleotide binding activity.