HOXA7 Colorimetric Cell-Based ELISA

A colorimetric cell-based ELISA kit from Assay Genie is designed for the accurate measurement of HOXA7 levels in cell lysates and culture supernatants. The kit uses a 96-well microplate format and a colorimetric detection method at 450 nm, and is reactive with human, mouse and rat samples. The stated dynamic range exceeds 5000 cells per well, and the kit is stored at 4°C for up to 6 months.

The assay operates as a lysate-free, high-throughput indirect ELISA, in which HOXA7-specific primary antibodies capture the target and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control to normalise target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density and adjust for plating differences.

Marketed by Assay Genie, the kit is intended for researchers studying gene expression, developmental biology and disease mechanisms. It can be used to measure relative amounts of HOXA7 in cultured cells and to screen the effects of various treatments, inhibitors or activators on HOXA7. The associated research area is epigenetics and nuclear signaling.