Horse IL-4 DIY ELISA Kit

The Assay Genie Horse IL-4 DIY ELISA Kit enables quantitative measurement of equine Interleukin-4, a key Th2 cytokine involved in allergic inflammation, parasite immunity, and regulatory T cell differentiation. Supplied as a reagent set sufficient for up to 10 microplates, the kit includes a pre-titrated capture antibody, biotinylated detection antibody, lyophilized recombinant equine IL-4 standard, and streptavidin-HRP conjugate; microplates are not included, letting laboratories pair the assay with their preferred coating buffer and plate type. The HRP-TMB signal is developed with chromogenic substrate and stopped with acid before absorbance is recorded on a standard plate reader. Researchers commonly apply this kit to equine samples harvested from models of asthma, recurrent airway obstruction, or inflammatory disease. Reconstituted standard and working detection antibody should be stored refrigerated and frozen in single-use aliquots to avoid repeated freeze-thaw cycles, while the remaining kit components are kept at 4 °C until first use. Each lot is validated by Assay Genie for specificity to native equine IL-4, with negligible cross-reactivity against related equine cytokines, ensuring signal reflects authentic IL-4 concentrations in horse samples.