Horse IL-12 p40 DIY ELISA Kit

Assay Genie's DIY ELISA format provides all the components needed to assemble custom immunoassays for equine IL-12 p40, including pre-titered capture and biotinylated detection antibodies, a lyophilised recombinant protein standard, streptavidin-HRP conjugate, TMB substrate, and stop solution. The kit supplies enough reagents to coat up to 10 microplates, with a 100 uL sample volume per well, supporting longitudinal equine immunology studies, vaccine response monitoring, and characterisation of Th1 pathway activity in infectious disease models such as Rhodococcus equi pneumonia. Because the p40 subunit pairs with both p35 to form IL-12 and p19 to form IL-23, measurements reflect both cytokine pools in clinical and experimental horse specimens. Researchers coat their own plates with the supplied capture antibody, then add samples, biotinylated detection antibody, and HRP conjugate sequentially. Reagents are stored refrigerated for short-term use and frozen for long-term stability, and the standard should be reconstituted just before plate setup to preserve binding activity.