Horse IL-1 alpha DIY ELISA Kit

The Horse IL-1 alpha DIY ELISA Kit from Assay Genie enables researchers to quantify Interleukin-1 alpha, a pro-inflammatory cytokine central to equine immune responses, in a do-it-yourself plate-coating format supplied by the user. IL-1 alpha drives fever, leukocyte recruitment, and synovial inflammation, making this assay valuable for studies of equine laminitis, osteoarthritis, endotoxemia, and exercise-induced muscle stress.

Each kit provides matched capture and detection antibody pairs, recombinant standard, and assay buffers for up to ten microplates, with a recommended 100 uL sample volume per well. The protocol supports synovial fluid, bronchoalveolar lavage, and conditioned culture media, and is validated for in vitro research use only. Store all reagents refrigerated at 4 degrees C; bring components to room temperature before running the assay, and avoid repeated freeze-thaw cycles of the reconstituted standard.

Typical applications include monitoring cytokine release in stimulated equine peripheral blood mononuclear cells, characterizing inflammatory kinetics in joint aspirates, and benchmarking anti-inflammatory interventions in veterinary research models.