Horse CD40 Ligand DIY ELISA Kit

CD40 Ligand (CD154) is a type II transmembrane glycoprotein expressed primarily on activated CD4+ T lymphocytes, platelets, and mast cells, where it engages CD40 on B cells, dendritic cells, and macrophages to drive immunoglobulin class switching, germinal centre formation, and pro-inflammatory cytokine release. This Assay Genie DIY ELISA kit enables researchers to quantify equine CD40L in cell culture supernatants and serum samples, supporting studies of equine vaccination response, immune-mediated disease models such as recurrent airway obstruction, and inflammatory disorders. Lyophilised components are supplied for assembly in the user's own laboratory, providing flexible plate-by-plate throughput and reduced waste for small-batch studies. Unopened reagents should be refrigerated and protected from light to preserve antibody binding activity through the stated shelf life. The assay has been validated to specifically detect equine CD40L with no significant cross-reactivity reported for unrelated TNF superfamily members under standard running conditions.