Horse 12-Hydroxyeicosatetraenoic acid (12HETE) ELISA Kit

This competitive ELISA kit from Assay Genie is designed for the accurate measurement of 12-Hydroxyeicosatetraenoic acid (12-HETE) levels in horse samples, including serum, plasma, and cell culture supernatants. 12-HETE is described as a key metabolite of arachidonic acid with diverse biological functions, including regulation of inflammation, cell proliferation, and vascular tone. Its dysregulation has been implicated in various diseases such as cancer, cardiovascular disorders, and inflammatory conditions, underscoring its importance as a potential diagnostic marker and therapeutic target.

The assay operates over a stated range of 0.156-10 ng/mL and is intended for research use only. Detection is colorimetric, with optical density read on a microplate reader set to 450 nm. Sample preparation guidance is provided for serum, plasma, urine, cerebrospinal fluid, cell culture supernatant, cell lysates, tissue homogenates, tissue lysates, and breast milk, each with specific centrifugation and storage conditions.

Overall, the kit offers researchers a reliable method for studying the role of 12-HETE in physiological and pathological processes, supporting a better understanding of disease mechanisms and the development of novel treatment strategies.