Homocysteine (Hcy) ELISA Kit

A high-quality, sensitive competitive ELISA kit from Assay Genie is designed for the quantitative detection of homocysteine (Hcy) in biological samples. Intended for research use only, the kit supports studies relating to cardiovascular disease, neurological disorders, and pregnancy complications by enabling accurate measurement of this amino acid in serum, plasma, and cell culture supernatants, with reliable and reproducible results.

The assay uses a competitive detection format with absorbance read at 450 nm and has a stated measuring range of 0.78-50 ng/ml. Reaction incubations with the detection reagents and substrate are performed at 37°C. The workflow involves sequential addition of standard or sample, detection reagents A and B, substrate solution, and stop solution, separated by wash steps.

Kit components include a dismountable ELISA microplate, lyophilized standard, sample diluent, assay diluents A and B, detection reagents A and B, wash buffer, substrate, stop solution, and plate sealer. Sample preparation guidance is provided for serum, plasma, urine, cerebrospinal fluid, cell culture supernatant, cell lysates, tissue homogenates, tissue lysates, and breast milk, with recommended centrifugation conditions and long-term storage guidance.