hnRNP K Colorimetric Cell-Based ELISA Kit

A cell-based ELISA kit from Assay Genie for the detection of hnRNP K (heterogeneous nuclear ribonucleoprotein K) protein expression in cultured cells, with reactivity against human, mouse and rat samples. The assay uses a colorimetric detection method read at 450 nm in a 96-well microplate format, with a stated dynamic range of > 5000 Cells. The kit is described as lysate-free, high-throughput and sensitive, and operates on an indirect ELISA format in which hnRNP K is captured by target-specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyse a colour reaction on substrate addition.

An anti-GAPDH monoclonal antibody is included as an internal positive control for normalisation of target absorbance, and a Crystal Violet whole-cell staining step is provided to determine cell density so that absorbance values can be adjusted for plating differences. The kit is intended for use with adherent cells and is suitable for measuring relative hnRNP K levels as well as screening treatments, inhibitors or activators (such as siRNA or chemicals) for their effects on hnRNP K expression. Storage is at 4 °C with a stated stability of 6 Months. The listed research area is Immunology.

The target is the HNRNPK gene product (Gene ID 3190, UniProt P61978), an RNA-binding protein involved in RNA processing and gene expression regulation, with a stated molecular weight of 48,562 Da. The kit is aimed at researchers studying RNA metabolism, gene regulation and RNA-binding proteins.