hnRNP C1/2 (Phospho-Ser260)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this colorimetric cell-based ELISA kit is designed for the qualitative detection of hnRNP C1/2 phosphorylation at Serine 260 directly in cultured cells. The kit uses an indirect ELISA format in which the target is captured by phospho-specific and total primary antibodies, with HRP-conjugated secondary antibodies catalysing a colorimetric reaction that is read at 450 nm. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, and a Crystal Violet whole-cell staining step is provided so that cell density can be factored into the final analysis.

The assay is reactive with human, mouse and rat samples and has a stated dynamic range of > 5000 Cells. Described as lysate-free and high throughput, the phospho-specific sub-type makes it suitable for screening the effects of treatments, inhibitors or activators on hnRNP C1/2 phosphorylation in adherent cells, supporting work in areas such as signal transduction and biomarker discovery, where phosphorylation at Ser260 has been implicated in cancer, neurodegenerative and metabolic conditions.

Database identifiers supplied with the product include Gene ID 3183, UniProt ID P07910, OMIM 164020 and the gene symbol HNRNPC, with the human ortholog located on chromosome 14q11.2.