Histone H3 (Phospho-Ser28)Colorimetric Cell-Based ELISA Kit

Assay Genie's cell-based ELISA kit is designed for the accurate detection of phosphorylated Ser28 on Histone H3, providing a sensitive and reproducible tool for researchers studying epigenetic modifications and gene expression regulation. Phosphorylation of Ser28 plays a crucial role in regulating transcriptional activity, with dysregulation of this modification linked to various diseases including cancer and neurological disorders.

The colorimetric detection method is read at 450 nm and the kit reacts with Human, Mouse, and Rat samples, with a dynamic range of > 5000 Cells. It functions as a lysate-free, high-throughput system that can detect Histone H3 protein phosphorylation and expression profiles in adherent cells. The kit is suitable for measuring relative amounts of phosphorylated Histone H3 in cultured cells and for screening the effects of various treatments, inhibitors (e.g., siRNA or chemicals), or activators on Histone H3 phosphorylation.

Qualitative determination is achieved through an indirect ELISA format in which Histone H3 (Phospho-Ser28) is captured by specific primary antibodies and detected via HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. To support reliable normalisation, a monoclonal antibody specific for human GAPDH is included as an internal positive control, and a Crystal Violet whole-cell staining method may be used to determine cell density.