Histone H2A.X (Phospho-Ser139)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for the accurate quantification of phosphorylated histone H2A.X (Ser139) levels in cell lysates and culture supernatants. The assay offers high sensitivity and specificity for reproducible results across a range of research applications.

Histone H2A.X is a critical component of the DNA damage response pathway, with phosphorylation at Ser139 serving as an important marker of DNA damage. This makes the kit a valuable tool for studying DNA repair, apoptosis, and cell cycle regulation, and for investigating cellular responses to genotoxic stress, cancer development, and potential therapeutic interventions.

The kit is a convenient, lysate-free, high-throughput and sensitive assay that detects Histone H2A.X protein phosphorylation and expression profile in cells. It can be used to measure the relative amounts of phosphorylated Histone H2A.X in cultured cells, as well as to screen for the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on H2A.X phosphorylation. Qualitative determination is achieved by an indirect ELISA format: Histone H2A.X (Phospho-Ser139) is captured by specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. For data normalisation, a monoclonal antibody specific for human GAPDH is included as an internal positive control, and the Crystal Violet whole-cell staining method can be used to determine cell density, allowing absorbance values to be normalised to cell amounts. The kit is reactive with Human, Mouse and Rat samples, with a dynamic range of > 5000 Cells and detection at 450 nm, and is suitable for adherent cell samples.