HGS Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit targets HGS in adherent cell samples and shows reactivity against human, mouse, and rat species. The colorimetric assay is read at 450 nm and has a dynamic range of greater than 5000 cells. Housed in a 96-well microplate format, the kit is designed as a lysate-free, high-throughput assay for detecting HGS protein expression profiles in cultured cells through an indirect ELISA format.

HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, while Crystal Violet whole-cell staining may be used to determine cell density and adjust for plating differences. The kit supports research areas including cell biology, angiogenesis, cancer, cardiovascular diseases, and neurodegenerative disorders. Storage is at 4°C for six months.