HER2 (Phospho-Tyr877)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed to quantify levels of phosphorylated HER2 at tyrosine 877 in cell lysates and tissue samples, supporting research into HER2 signaling pathways relevant to cancer biology and drug development. The kit detects HER2 protein phosphorylation and expression profile in cells using a lysate-free, high-throughput indirect ELISA format, making it suitable for measuring the relative amounts of phosphorylated HER2 in cultured cells and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on HER2 phosphorylation.

Primary antibodies specific for HER2 (Phospho-Tyr877) capture the target, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling a colorimetric reaction upon substrate addition that is read at 450 nm. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density. The kit is reactive with Human, Mouse, and Rat samples, with adherent cells as the indicated sample type, and has a dynamic range of > 5000 Cells.

Associated database identifiers include Gene ID 2064, UniProt ID P04626, OMIM 164870, Unigene Hs.446352, and Gene Symbol ERBB2. The sub-type is Phospho, and HER2 is described as a proto-oncogenic receptor tyrosine kinase of the EGFR family, with the gene amplified or overexpressed in up to 30% of breast cancers and somatic mutations seen in 4% of lung cancers, as well as in breast, gastric, ovarian cancer, and glioblastoma.