HER2 (Phospho-Tyr1248)Fluorometric Cell-Based ELISA Kit

Assay Genie's Fluorometric Cell-Based ELISA Kit targets phosphorylated HER2 at the Tyr1248 residue, offering a convenient, lysate-free, high throughput and sensitive approach for monitoring HER2 phosphorylation and the expression profile of phosphorylated HER2 in cultured cells. It enables qualitative measurement of relative phosphorylated HER2 (Phospho-Tyr1248) levels and supports screening of the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on HER2 phosphorylation.

The kit uses an indirect ELISA format in which HER2 (Phospho-Tyr1248) is captured by phospho-specific primary antibodies, with Dye 1- and Dye 2-conjugated secondary antibodies binding the Fc region of the primary antibody for fluorometric detection. Two normalization approaches are included: a monoclonal antibody specific for human GAPDH as an internal positive control, and an antibody against the nonphosphorylated counterpart of HER2 (Phospho-Tyr1248), whose RFU values can be used to normalize those of phosphorylated HER2.

The assay is reactive with Human, Mouse, and Rat samples, has a dynamic range of > 5000 Cells, and is read with a fluorescent plate reader at Ex/Em 651/667 (Dye 1) and 495/521 (Dye 2). The target is reported under NCBI Gene Symbol ERBB2 (Gene ID: 2064, UniProt ID: P04626, OMIM: 164870, Unigene: Hs.446352). The kit is stored at 4°C with a stability of 6 Months.