HER2 (Phospho-Tyr1221/Tyr1222)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the detection and quantification of phosphorylated HER2 at tyrosine residues 1221 and 1222 in cell-based systems, with a dynamic range of > 5000 Cells. The colorimetric assay, read at 450 nm, uses an indirect ELISA format in which phospho-specific primary antibodies capture the target and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition, enabling qualitative measurement of HER2 phosphorylation in cultured cells in response to different stimuli, drug treatments, or genetic manipulations.

Reactive with human, mouse, and rat samples and intended for adherent cell cultures, the assay is described as lysate-free and high throughput. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining method is provided to determine cell density so that absorbance readings can be adjusted for plating differences. Phosphorylation of HER2 at tyrosines 1221 and 1222 is a key regulatory event in this EGFR-family receptor tyrosine kinase, and dysregulation of HER2 signalling has been implicated in cancers such as breast cancer, where the gene is amplified or overexpressed in up to 30% of cases.