HDAC8 Colorimetric Cell-Based ELISA Kit

Assay Genie's colorimetric cell-based ELISA kit is designed for the qualitative detection of HDAC8 protein expression in cultured cells. The assay uses an indirect ELISA format in which HDAC8 is captured by target-specific primary antibodies; HRP-conjugated secondary antibodies then catalyse a colorimetric reaction upon substrate addition, with absorbance read at 450 nm.

Reactive with human, mouse and rat samples, with a stated dynamic range of > 5000 cells, the kit is suited to measuring relative HDAC8 amounts in cultured cells and to screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on HDAC8. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density after the colorimetric measurement. Storage is recommended at 4°C with a stated stability of 6 months. The product is positioned for research in epigenetics and nuclear signaling.

HDAC8 (Gene ID 55869, UniProt Q9BY41) is a class I histone deacetylase that catalyses deacetylation of lysine residues on the N-terminal tails of the core histones H2A, H2B, H3 and H4, and has been associated with transcriptional regulation, cell cycle progression, and Wilson-Turner X-linked mental retardation syndrome.