HDAC7 Colorimetric Cell-Based ELISA Kit

A cell-based ELISA kit from Assay Genie designed for the qualitative detection of HDAC7 protein levels in cultured cells. The assay uses an indirect ELISA format in which HDAC7 is captured by target-specific primary antibodies, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. The colorimetric signal is measured at 450 nm.

Key specifications include: target HDAC7; ELISA type Cell-Based; reactivity Human and Mouse; dynamic range > 5000 Cells; detection method Colorimetric at 450 nm; format 96-Well Microplate; storage at 4°C for 6 Months; and sample type Adherent Cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density and adjust for plating differences.

HDAC7 is a member of the histone deacetylase family responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), a modification that tags chromatin for epigenetic repression and contributes to transcriptional regulation, cell cycle progression, and developmental events. The kit is intended for research applications in molecular biology, drug discovery, and personalised medicine, within the research area of Epigenetics and Nuclear Signaling.