HDAC5 (Phospho-Ser498)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA kit is designed for the detection of HDAC5 protein phosphorylation at Ser498 in cultured cells. It is a lysate-free, high-throughput and sensitive assay that uses an indirect ELISA format: HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, and the conjugated HRP enzyme catalyses a colorimetric reaction at 450 nm upon substrate addition. The assay has a dynamic range of > 5000 Cells and is reactive with Human and Mouse samples, with adherent cells as the indicated sample type.

Phosphorylation of HDAC5 at Ser498 has been implicated in cellular pathways relevant to cancer, cardiovascular disease and neurodegenerative disorders, making it a target of interest in cell signalling and molecular biology research. The kit is suitable for measuring the relative amounts of phosphorylated HDAC5 in cultured cells, and for screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on HDAC5 phosphorylation.

Because the Cell-Based ELISA is qualitative, two normalisation methods are described: a monoclonal antibody specific for human GAPDH serves as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step is used to determine cell density so that absorbance values can be adjusted to cell amounts.