HDAC5 (Phospho-Ser259)Fluorometric Cell-Based ELISA Kit

Manufactured by Assay Genie, this Phospho Cell-Based ELISA Kit is designed to monitor HDAC5 phosphorylation and expression profiles in cultured cells using a fluorometric detection method. The lysate-free, indirect ELISA workflow captures the phosphorylated target with a specific primary antibody, then uses Dye 1- and Dye 2-conjugated secondary antibodies to enable fluorescent readout on a plate reader with two channels at Ex/Em of 651/667 nm and 495/521 nm.

The assay is reactive with human and mouse samples and is intended for measuring relative levels of phosphorylated HDAC5 (Ser259) in cells, as well as screening the effects of treatments, inhibitors such as siRNA or chemicals, and activators on HDAC5/HDAC9 phosphorylation. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, alongside an antibody against the non-phosphorylated counterpart of the target. The reported dynamic range is greater than 5000 cells, and the kit is intended for storage at 4°C with a stability of 6 months.