HDAC4 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit provides a convenient, lysate-free, high-throughput method for measuring HDAC4 protein expression in cultured cells. The assay works on adherent samples and is reactive with human, mouse, and rat material. It is supplied in a 96-well microplate format with colourimetric detection at 450 nm and has a stated dynamic range of > 5000 cells. Storage is at 4°C with a stability of 6 months.

The kit uses an indirect ELISA format in which HDAC4-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, allowing the conjugated HRP to catalyse a colourimetric reaction upon substrate addition. It can be used to quantify relative HDAC4 levels in cultured cells as well as to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on HDAC4. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step is provided for determining cell density so that absorbance values can be normalised to cell amounts.

HDAC4 (histone deacetylase 4) is a transcriptional regulator of the histone deacetylase family, subfamily 2, that deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 and H4. It plays a role in transcriptional regulation, cell cycle progression and developmental events and does not bind DNA directly but through transcription factors MEF2C and MEF2D. Dysregulation of HDAC4 has been implicated in cancer, neurodegenerative disorders and cardiovascular diseases, making accurate measurement of HDAC4 levels important for studies of disease pathogenesis and therapeutic interventions.