HDAC2 (Phospho-Ser394)Colorimetric Cell-Based ELISA Kit

Assay Genie's phospho-specific cell-based ELISA kit is designed for the precise detection of HDAC2 phosphorylation at serine 394 in cell samples, using a colorimetric detection method with measurement at 450 nm. The assay is reactive with human, mouse, and rat samples, has a dynamic range of > 5000 cells, and is intended for use with adherent cells.

Operating as a lysate-free, high-throughput, cell-based ELISA in an indirect format, the kit captures phospho-Ser394 HDAC2 with target-specific primary antibodies, which are then bound by HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density for further normalization against plating differences.

The kit enables qualitative measurement of phosphorylated HDAC2 in cultured cells, supporting studies of the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on HDAC2 phosphorylation, which is relevant to research areas including cancer, neurodegenerative disorders, and inflammatory conditions.