HBP1 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit supports the quantitative measurement of HBP1 (HMG box-containing protein 1) in cell samples using a colorimetric detection method at 450 nm. The assay uses a 96-well microplate and demonstrates reactivity with human, mouse, and rat samples, with a dynamic range of "> 5000 Cells". Storage is specified at 4°C with a stated stability of 6 months.

HBP1 is characterized in the UniProt entry (O60381) as a transcriptional repressor that binds to the promoter region of target genes, with documented roles in the regulation of the cell cycle and of the Wnt pathway. The chromosomal location of the human ortholog is 7q22.3, and the molecular weight is listed as 57,645 Da. The indirect ELISA format employs HBP1-specific primary antibodies together with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. An anti-GAPDH monoclonal antibody is included to serve as an internal positive control for normalizing target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density. The workflow is described as lysate-free and high throughput, suitable for measuring relative HBP1 amounts in cultured cells and for screening the effects of treatments, inhibitors, or activators on HBP1 expression.