Hamster IFN-gamma (Interferon gamma) ELISA Kit

The Hamster IFN-gamma (Interferon gamma) ELISA Kit from Assay Genie is a sandwich-format immunoassay built on a 96-well microplate for quantitative measurement of interferon-gamma in hamster serum and plasma. IFN-gamma is a hallmark Th1 cytokine that drives macrophage activation, antigen presentation, and antiviral defense, making it a key readout in hamster infectious disease, inflammation, and vaccine research, including SARS-CoV-2, Leishmania, and tuberculosis challenge models that use the golden Syrian hamster as a translational system. The assay uses a matched capture and detection antibody pair with streptavidin-HRP and reports a detection range of 15.6 to 1000 pg/mL with a sensitivity under 5 pg/mL, requiring only 100 µL of sample per well. A seven-point standard curve is provided with TMB substrate for color development at 450 nm. Reagents are ready to use in a single kit box, and the components should be stored at 2-8°C to preserve activity and avoid repeated freeze-thaw cycles. This research-use-only kit supports cytokine profiling in vaccine efficacy studies, immune response characterization, and comparative immunology work where IFN-gamma serves as a quantitative biomarker of cell-mediated immunity.