Guinea pig Ubiquitin (Ub) ELISA Kit

Ubiquitin is a 76-amino-acid (~8.5 kDa) regulatory protein that covalently tags cellular substrates for proteasomal degradation. Quantitative measurement of ubiquitin in guinea pig specimens is supported by the Ubiquitin (Ub) ELISA Kit from Assay Genie, which enables researchers to investigate altered ubiquitin homeostasis in guinea pig disease models of neurodegeneration, inflammation, and muscle wasting, where dysregulated ubiquitin-proteasome signaling has been documented.

Each kit provides reagents sufficient for 96 individual determinations and has been validated across multiple sample matrices, including tissue homogenates, cell lysates, cell culture supernatants, and additional specimen types from guinea pig. The assay is calibrated to specifically detect guinea pig ubiquitin, an important consideration when working with guinea pig disease models where ubiquitin pathway dysregulation is being characterized.

For storage, unopened kit components should be kept refrigerated. Reconstituted standards and the working conjugate are best preserved by aliquoting to limit repeated freeze-thaw cycles. Standard curves are constructed from the included calibrators, absorbance is recorded on a standard microplate reader, and each run typically includes overnight incubation steps followed by chromogenic development.