Guinea pig TIMP-1/Tissue Inhibitors of Metalloproteinase 1 ELISA Kit

The Assay Genie sandwich ELISA kit is designed for the quantitative measurement of TIMP-1 (Tissue Inhibitors of Metalloproteinase-1) in guinea pig samples. It uses a double antibody sandwich ELISA format with colorimetric detection and is suitable for serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. The kit is intended for research applications related to tissue remodeling, inflammation, and cancer progression, where TIMP-1 serves as a regulator of the extracellular matrix and a valuable biomarker.

Key performance specifications include a sensitivity of 2.813 pg/mL and a standard range of 4.688–300 pg/mL. The kit should be stored at 2–8°C for 12 months. The assay procedure involves a primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, and TMB color development in the dark for 10–20 minutes, with absorbance read at 450 nm immediately after the stop reaction.