Guinea pig Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit

This colorimetric kit from Assay Genie targets Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES), also known as CCL5, a C-C chemokine central to leukocyte recruitment, T-cell signaling, and inflammatory cascades. In guinea pig models—widely used for respiratory allergy, tuberculosis, and anaphylaxis studies—quantifying RANTES helps researchers track early immune activation and chemotactic gradients across a panel of biological fluids.

The assay runs on a pre-coated microplate strip format with all reagents supplied for a complete run. Researchers load 50 µL of sample per well alongside a standard dilution series, generating a standard curve to interpolate unknown concentrations. Reconstituted reagents should be stored at -20 °C to preserve activity, with unused strip wells returned to the desiccated pouch and sealed to protect from moisture. Reconstituted standards and detection antibody should be aliquoted to limit freeze-thaw cycles.

Guinea pig-specific capture and detection antibodies minimize cross-reactivity with related chemokines such as MIP-1α or MCP-1, supporting clean measurement of endogenous RANTES in vaccination, asthma, and infectious disease workflows. Intended for research use only, the kit includes a wash buffer concentrate, substrate solution, and stop solution sufficient for the full 96 assays.