Guinea pig PEDF/Pigment Epithelium Derived Factor ELISA Kit

This Sandwich ELISA Kit from Assay Genie is designed for the accurate detection of PEDF (Pigment Epithelium-Derived Factor) levels in guinea pig samples. The assay uses a double antibody sandwich format with colorimetric detection, and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types.

Performance specifications include a sensitivity of 18.75 pg/mL and a measurable range of 31.25–2000 pg/mL. Reported linearity spans 80–105% across serial dilutions of serum, EDTA plasma, and heparin plasma, while spike-recovery averages 90% in serum, 92% in EDTA plasma, and 95% in heparin plasma. The kit is stored at 2–8°C for 12 months and includes a lyophilized standard, biotin-labeled antibody, HRP-streptavidin conjugate, TMB substrate, and assay buffers. PEDF is recognized for its anti-angiogenic and neurotrophic properties, with dysregulation linked to cancer, diabetes, and retinal degeneration.

The workflow involves a 90-minute primary incubation at 37°C, followed by biotinylated detection antibody and HRP-streptavidin binding steps, with TMB color development read at 450 nm. Synonyms covered by the kit include Serpin F1, EPC-1, and Cell prolifeRation-inducing gene 35 protein; the associated UniProt entry is A7L6D3.