Guinea pig Interleukin 2 (IL-2) ELISA Kit

Interleukin-2 (IL-2) is a key immunoregulatory cytokine governing T lymphocyte proliferation, NK cell cytotoxicity, and regulatory T cell maintenance. This Assay Genie kit provides a sandwich ELISA platform specifically validated for quantitative detection of guinea pig IL-2, with a detection range of 31.2 to 2000 pg/mL and analytical sensitivity near 18.75 pg/mL. The protocol uses a 100 µL sample volume per well of the pre-coated microplate, with absorbance read at 450 nm and results calculated against a standard curve. Unopened reagents are stored at 4°C, while reconstituted standards and detection antibody should be aliquoted and kept frozen to limit freeze-thaw loss. The assay demonstrates minimal cross-reactivity with mouse, rat, and human IL-2, supporting its use in vaccine adjuvant screening, infectious disease modeling, delayed-type hypersensitivity testing, and transplantation research where the guinea pig serves as the primary animal model.