GTPase Activating Protein (Phospho-Ser387)Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this Cell-Based ELISA Kit provides a colorimetric, lysate-free assay for measuring phosphorylated GTPase Activating Protein at Ser387 in adherent cell samples. The kit employs an indirect ELISA format in which a target-specific primary antibody is captured and detected using HRP-conjugated secondary antibodies, with signal generated at 450 nm.

Validated for use with Human, Mouse, and Rat samples, the assay has a dynamic range of > 5000 Cells and is intended for cell cycle research. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and Crystal Violet whole-cell staining can be used to determine cell density and adjust for plating differences. The underlying target, RACGAP1, encodes a Rac GTPase-activating protein implicated in cytokinesis and Rho-mediated signaling.

The kit enables qualitative measurement of relative phosphorylated GTPase Activating Protein levels in cultured cells, supporting studies of the effects of various treatments, inhibitors (such as siRNA or chemicals), and activators on GTPase Activating Protein phosphorylation.