GRB10 (Phospho-Tyr67)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA kit is designed to detect the phosphorylation of GRB10 at tyrosine 67 in cultured cells. The colorimetric assay uses a 450 nm detection method and is reactive with human samples, with a dynamic range of > 5000 Cells. It is a convenient, lysate-free, high-throughput and sensitive assay for measuring the relative amounts of phosphorylated GRB10 in cultured cells, as well as for screening the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on GRB10 phosphorylation.

Detection is achieved through an indirect ELISA format, where target-specific primary antibodies capture the analyte and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density, allowing results to be normalised to cell amounts and plating differences to be adjusted. GRB10 is a key adaptor protein involved in cell growth, differentiation, and metabolism, with phosphorylation at tyrosine 67 implicated in conditions such as cancer, diabetes, and obesity.