Goat Interleukin 1alpha (IL-1alpha) ELISA Kit (GTFI00035)

This cytokine quantification ELISA from Assay Genie targets caprine IL-1alpha, a pro-inflammatory mediator released during innate immune activation, microbial infection, and tissue injury in ruminants. The kit uses a matched antibody pair immobilized on a microplate to capture IL-1alpha from prepared specimens, with detection range 15.6 to 1000 pg/mL and sensitivity less than 5 pg/mL. Researchers studying mastitis, endotoxemia, vaccine responses, or reproductive inflammation in dairy and meat goats typically load 100 uL of clarified serum or EDTA plasma per well, interpolating unknowns against a recombinant goat IL-1alpha standard curve. Each pack contains a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, chromogenic substrate, stop solution, sealed vials of calibrator, and an instruction booklet. Store unopened components at -20°C; equilibrate working reagents to ambient temperature before pipetting, and limit reconstituted standards to no more than three freeze-thaw cycles to preserve immunoreactivity.