Glutathione (GSH) ELISA Kit

This Competitive ELISA Kit from Assay Genie is designed for the quantitative measurement of glutathione (GSH) in research samples. Glutathione is a vital antioxidant molecule that protects cells from oxidative stress, and altered glutathione levels have been implicated in conditions including cancer, liver diseases, and neurodegenerative disorders, making reliable measurement valuable for studies of oxidative stress and cellular health.

The assay uses a competitive enzyme immunoassay format with detection at 450 nm and a working range of 1.25–80 ng/mL. It has been validated for a broad panel of sample types including serum, plasma, cell culture supernatants, urine, cerebrospinal fluid, cell lysates, tissue homogenates, tissue lysates, and breast milk. The manufacturer describes the kit as offering high sensitivity and specificity with consistent, precise results, and the product is intended for research use only.

Included components are a dismountable ELISA Microplate, a lyophilized standard, sample diluent, Assay Diluent A, Assay Diluent B, Detection Reagent A, Detection Reagent B, wash buffer, substrate solution, stop solution, and plate sealers, with most components stored at –20 °C and the wash buffer, substrate solution, and stop solution stored at 4 °C. Sample-preparation protocols are provided for serum, plasma, urine, cerebrospinal fluid, cell lysates, tissue homogenates and lysates, breast milk, and cell culture supernatants.