GluR2 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for detecting and quantifying GluR2 in cell samples using an In-Cell, lysate-free protocol. The assay is read colorimetrically at 450 nm, and the kit is supplied in a 96-Well Microplate format with a stated stability of 6 Months at 4°C. It is intended for adherent cell samples and shows reactivity with Human, Mouse, and Rat, with a dynamic range of > 5000 Cells.

The kit uses an indirect ELISA format, capturing GluR2 with target-specific primary antibodies and detecting it with HRP-conjugated secondary antibodies that catalyse a colour reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising absorbance values, and a Crystal Violet whole-cell staining step can be used to determine cell density and adjust for plating variation. The kit can be applied to measure relative GluR2 levels in cultured cells and to screen the effects of treatments, inhibitors such as siRNA or chemicals, and activators on GluR2 expression. The target, GluR2 (gene symbol GRIA2, Gene ID 2891, UniProt ID P42262), is a subunit of the AMPA receptor, and the kit is listed under the Signal Transduction research area.