GluR1 (Phospho-Ser863)Colorimetric Cell-Based ELISA Kit

Designed for the accurate measurement of GluR1 phosphorylation at serine 863 in cell samples, this Cell-Based ELISA Kit from Assay Genie is a lysate-free, high-throughput and sensitive assay that uses an indirect ELISA format. Target-specific primary antibodies capture GluR1 (Phospho-Ser863), and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. The kit is intended for measuring relative phosphorylated GluR1 in cultured cells and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals) or activators on GluR1 phosphorylation. The dynamic range is stated as > 5000 Cells.

The detection method is colorimetric, read at 450 nm, with reactivity established for Human, Mouse and Rat samples. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising the target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density so that absorbance values can be normalised to cell amounts. The research area is Signal Transduction and the sub-type is Phospho, supporting work on the AMPA-receptor subunit GluR1 (GRIA1) and its role in excitatory neurotransmission and synaptic plasticity.