GK3 Colorimetric Cell-Based ELISA

A cell-based ELISA kit from Assay Genie for the qualitative detection of GK3 (Glycerol kinase 3) protein expression in cultured cells. The assay uses an indirect ELISA format in which GK3-specific primary antibodies are bound by HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. The kit is lysate-free and suited to high-throughput screening of treatments, inhibitors (such as siRNA or chemicals), or activators on GK3 levels.

Designed for adherent cell samples, the colorimetric readout is measured at 450 nm and supports a dynamic range of > 5000 cells. Normalisation is achieved with an included anti-GAPDH antibody as an internal positive control, together with an optional Crystal Violet whole-cell staining step for determining cell density. GK3 (Gene ID 2713, molecular weight 60,598 Da) is described as a key enzyme in the regulation of glycerol uptake and metabolism. The kit is relevant to research areas including Epigenetics and Nuclear Signaling and Signal Transduction. Storage is at 4°C for 6 months.

Supplied components include 96-well cell culture clear-bottom microplates, 10X TBS, Quenching Buffer, Blocking Buffer, 15X Wash Buffer, Primary Antibody Diluent, 100x Anti-Phospho Target Antibody, 100x Anti-Target Antibody, Anti-GAPDH Antibody, HRP-Conjugated Anti-Rabbit IgG Antibody, HRP-Conjugated Anti-Mouse IgG Antibody, SDS Solution, Stop Solution, Ready-to-Use Substrate, Crystal Violet Solution, and Adhesive Plate Seals.