General Vitamin D2 (VD2) ELISA Kit

This competitive ELISA from Assay Genie quantifies 25-hydroxyvitamin D2 (25-OH-D2), the principal circulating form of ergocalciferol obtained from dietary intake and supplementation. Researchers deploy the assay in studies of nutritional status, bone metabolism, calcium homeostasis, and the effectiveness of vitamin D2 supplementation, including work on plant-based dietary patterns and fortified food formulations. The protocol uses competitive binding between sample analytes and coated antigen, with a horseradish peroxidase-labelled detection antibody generating a colorimetric signal that decreases as vitamin D2 concentration rises. Each pack contains a pre-coated microplate, calibration standards, detection antibody concentrate, wash buffer, substrate solution, stop reagent, and a step-by-step protocol insert. Recommended sample volume is 50 uL, and all reagents should be refrigerated between uses to preserve binding activity and shelf life. For research use only.