General Triiodothyronine (T3) ELISA Kit

Quantitative measurement of total triiodothyronine in research specimens is achieved through competitive immunoassay, where analyte in the sample competes with a labeled conjugate for binding sites on an immobilized antibody. This Assay Genie kit is supplied as a 96-assay microplate format and uses a colorimetric endpoint read by standard laboratory plate readers. The procedure accepts serum, heparinized plasma, and similar liquid samples, with reactivity characterized as universal across mammalian species.

Each package contains pre-coated wells, a set of calibrators spanning the expected working range, enzyme conjugate, wash concentrate, substrate, and stop solution, plus a detailed protocol. Store reagents refrigerated at 4°C until first use, allow kit components to equilibrate to room temperature before running the assay, and avoid repeated freeze-thaw cycles of working dilutions. The competitive format is well matched to small-molecule hormones and supports studies in thyroid physiology, metabolic regulation, veterinary endocrinology, and pharmacology research.

Provided for research use only, the kit is not cleared for diagnostic procedures. Specimens should be diluted as directed in the package insert, and absorbance values should be recorded after the enzymatic reaction is stopped with the supplied reagent.