General Tetrahydrobiopterin (THB) ELISA Kit

The Assay Genie General Tetrahydrobiopterin (THB) ELISA Kit is a quantitative immunoassay for measuring BH4, an essential pterin cofactor required by phenylalanine hydroxylase, tyrosine hydroxylase, tryptophan hydroxylase, and all three nitric oxide synthase isoforms. Because BH4 is also a recognized marker of oxidative stress, the kit is widely used in cardiovascular, neurological, and metabolic disease research, including studies of phenylketonuria, DOPA-responsive dystonia, endothelial dysfunction, and biopterin metabolism disorders. The competitive format enables direct measurement of the small pteridine molecule, requiring a 50 uL sample volume per well, and the kit is supplied with pre-coated microplate wells, detection antibody, calibrated standards, chromogenic substrate, and wash buffer. Investigators probing monoaminergic neurotransmitter synthesis, reactive oxygen species pathways, or nitric oxide biology gain a reliable quantitative tool for serum, plasma, and tissue homogenate samples. Reagents should be stored refrigerated at 4 degrees C, and the kit is intended for research use only.