General Reverse Triiodothyronine (rT3) ELISA Kit

Quantification of 3,3',5'-triiodothyronine (rT3), the inactive isomer generated by inner-ring deiodination of thyroxine (T4), supports research into altered thyroid hormone metabolism in non-thyroidal illness, caloric restriction, hepatic and renal dysfunction, and modified type 3 deiodinase activity. The Assay Genie reverse triiodothyronine (rT3) ELISA Kit is configured as a competitive immunoassay because rT3 is a small hapten-like molecule that cannot be sandwiched between two non-overlapping antibodies.

The assay uses competition between sample rT3 and an rT3-enzyme conjugate for limited antibody binding sites on pre-coated plate wells, with a colorimetric endpoint readable on standard microplate spectrophotometers. The 96-assay kit supplies reagents for moderate-throughput profiling across human, rodent, and livestock sample matrices in in vitro research applications.

Package contents include pre-coated plate wells, calibrator standards, assay buffer, conjugate, substrate, and stop solution, with a complete protocol provided in the insert. The workflow requires standard laboratory equipment such as a plate washer and a microplate reader capable of measuring absorbance.