General Prostaglandin F2 Alpha (PGF2a) ELISA Kit

Prostaglandin F2 Alpha (PGF2α) is a bioactive lipid mediator synthesized from arachidonic acid via the cyclooxygenase pathway, with established roles in reproductive biology, luteolysis, uterine contraction, ocular pressure regulation, and inflammatory signaling. Research laboratories investigating these pathways depend on quantitative immunoassays to measure PGF2α concentrations in experimental samples.

This competitive ELISA from Assay Genie is supplied as a 96 assays kit with an absorbance-based readout compatible with standard microplate readers. Each kit includes the pre-coated plate, calibrated reference standards, specific antibody, and assay buffers needed to construct a standard curve and quantify unknowns in a single plate run. Intended for research use only, the assay supports pharmacodynamic studies, reproductive physiology investigations, inflammatory response characterization, and agricultural or veterinary research where PGF2α functions as a relevant biomarker.

Workflow involves adding standards and samples to the precoated wells, completing the competitive binding step, and reading the plate after substrate addition. Results are interpolated from the standard curve to determine PGF2α concentration in each sample. The kit ships in a refrigerated pack and should be stored according to the enclosed insert to maintain reagent integrity across the stated shelf life. The microplate configuration supports routine throughput, allowing standard curves and experimental samples to be processed simultaneously for reproducible data across research teams.