General Prostaglandin E2 (PGE2) ELISA Kit

Quantify Prostaglandin E2 (PGE2), a major arachidonic acid-derived lipid mediator, using this competitive immunoassay kit from Assay Genie. PGE2 is generated through cyclooxygenase (COX-1 and COX-2) pathways and serves as a central regulator of inflammation, fever, vascular permeability, pain perception, and reproductive function.

The kit operates on a competitive ELISA format with a colorimetric readout, providing an alternative to LC-MS workflows for laboratories studying eicosanoid signaling. Each pack contains a pre-coated microplate, PGE2 standards, enzyme conjugate, substrate solution, and wash buffer sufficient for 96 assays.

Researchers in inflammation biology, oncology, reproductive medicine, and pharmacology apply this assay to monitor COX pathway activity in cell culture models, evaluate NSAID or selective COX-2 inhibitor effects, and characterize disease-associated eicosanoid profiles in tissue extracts.

Specimens processed include serum-derived fractions, plasma-derived fractions, cell culture supernatant, and tissue homogenates. A small uL sample volume is required per well, and absorbance readings are taken on a standard microplate reader. Reagents should be refrigerated at 4 degrees C between uses, and the kit is labeled for research use only.