General Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit

Quantifying intracellular calcium-mobilizing second messengers is fundamental to research on endolysosomal signaling and organellar messenger dynamics, and NAADP (nicotinic acid adenine dinucleotide phosphate) sits at the center of that work as a pyridine nucleotide messenger. This competitive immunoassay from Assay Genie is supplied as a 96-assay kit configured around a universal reactivity design, enabling detection across diverse sample matrices including cell lysates, tissue extracts, and conditioned media. Investigators characterizing calcium release channels, two-pore channel behavior, and messenger-metabolite crosstalk apply this plate-based workflow to map NAADP fluctuations during cellular stimulation or pharmacological intervention. The protocol is validated for in vitro research use only and produces a signal compatible with routine microplate spectrophotometer readers, integrating easily into standard life-science laboratory pipelines. Each kit ships with reagents prepared for the full assay count and includes the buffers and standards required to generate a quantitative standard curve.