General Glucocorticoid (GC) ELISA Kit

Glucocorticoids are steroid hormones that regulate stress response, glucose metabolism, immune function, and inflammation, making accurate quantitation central to endocrinology and metabolism research. The Assay Genie General Glucocorticoid (GC) ELISA Kit delivers a competitive immunoassay platform for measuring these hormones across a broad spectrum of research samples.

Built on a competitive ELISA format, the kit produces an inverse signal in which higher glucocorticoid concentrations in the sample correspond to lower absorbance readings. This configuration is well matched to small steroid analytes that cannot be paired in a sandwich layout. The colorimetric endpoint is captured on a standard microplate reader, and each kit is sized for 96 assays, accommodating both small pilot studies and larger screening panels.

Validated with universal reactivity, the assay is not restricted to a single species and can be deployed across mammalian cell culture work, rodent tissue homogenates, and cell lysates harvested from in vitro experiments. Acceptable sample matrices include cell culture supernatants, tissue homogenates, and cell lysates, allowing researchers to profile glucocorticoid output from adrenal, hepatic, or neural sources.

Each kit ships with a pre-coated microplate, lyophilized reference standards, enzyme-conjugated detection reagents, and a complete buffer set. Intended for research use only, the assay supports investigations of hypothalamic-pituitary-adrenal (HPA) axis activity, cortisol and corticosterone regulation, and stress-related metabolic signaling in both basic and translational workflows.